Western Blotting

Dot and Slot Blot

Colony Hybridization and Plaque Lift Assay

Northern Blotting/ Hybridization

Blotting Techniques

Southernwestern Blotting

Optimization of Codon/Codon Choice,mRNA structure:Factor Affecting cloned gene Expression in E. coli

Plasmid stability and plasmid copy number:Structural and segregative stability,incompatibility

Optimization of Translational initiation sequences 5՚UTR for cloned gene in E.coli

Transcriptional Termination Choice for expression Vectors in E.coli

Promoter Strength:lacUV5,hybrid promoters:tac,tic:factors affecting cloned gene expression in E.coli

Response element :Eukaryotic Gene Expression Regulation

Difference between pUC18 and pUC19/Compare and Contrast:pUC18 and pUC19/high copy no. plasmid vector

Alpha Complementation, Blue White selection, pUC18

Two Hybrid System, Two Hybrid Assay,Yeast Hybrid System,Method to detect protein protein interaction

DNA Binding Domain, Activating Region: Zinc Finger,Leucine Zipper,Helix Loop Helix,Helix Turn Helix

E.coli Expression Vectors: Cloned Gene Under Control of Regulated Promoter: λPL,T7, pBAD, pET vector

Global Regulators/Global control system/Global control Network/Global Regulatory System

gal operon: Structure, Regulation, two levels of regulation, GalR

Isolation of Bacterial Genomic DNA: Steps for isolation, methods to purify and concentrate DNA

Finding Concentration of DNA and Purity Check of Isolated DNA

Plasmid DNA Isolation Methods:Alkaline lysis, EtBr-CsCl density gradient, Isolation on basis of Size

Real time PCR/ Quantitative PCR/ RT-PCR/ qPCR

Inverse PCR : Variation of PCR / Types of PCR

Primer Designing for PCR

Polymerase Chain Reaction/PCR Basic Principle/ Essential Components of PCR/Parameters Affecting PCR

Genetic / Genomic Imprinting: Importance in Epigenetics, When and How imprinting happens: Disorders

No Go Decay pathway: Dom34/Hbs1, Pelota/Hbs1, Pelota in Begomovirus resistance in Tomato, ty5 locus

Chromatin remodeling complexes/Antirepressor, HO gene & PHO5 gene Activation

Histone Modification: Acetylation, Deacetylation, Methylation Demethylation of Histones