This method of making re-usable agar plates is super easy and reliable. You don't need a laminar flow hood or still air box to pour the agar plates because they are poured before sterilization. The twist top eliminates the need for parafilm around the edges. I have not noticed any problems from lack of air exchange for the short term. If you plan on using them for longer term storage i would loosen the lid and wrap in micropore tape or parafilm.

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