Tutorial: How to organize unstained controls within SpectroFlo (Cytek Aurora) in order to utilize the universal negative and/or autofluorescence extraction features. This video also discusses how to deal with multiple unstained controls when using multiple tissues in the same experiment.

Video Contents
0:00 Unstained controls - purpose and requirements
4:16 Example experiments for universal negative feature
5:24 Tutorial for universal negative feature
10:58 Example experiments for autofluorescence extraction feature
11:54 Tutorial for autofluorescence extraction feature