Here is the link to the three files: https://gtvault-my.sharepoint.com/:u:...

The tutorial video that takes you through how to use PHENIX Autobuild, PHENIX Refine, and Coot, for STARS Atlanta Branch handout September 30th, 2024.

After following along with the tutorial video, you should be able to find the source .mtz and .fasta files on the PDB as well as know where to find the original R-work, R-free, and resolution values for the electron density map. You should also be able to understand how PHENIX.autobuild works as well as be able to run PHENIX.refine and Coot iteratively until the R-work and R-free values begin decreasing. You should also be able to compare your refined structure results to that of the original PDB structure in PyMol.