Hey Friends,

Transformation, Conjugation and Transduction are ways of bacteria to transfer genetic material horizontally. Transformation is the uptake of free genetic material. Conjugation is exchange of DNA via cell-cell contact and Transduction is gene transfer via a viral vector. In the lab, we use Heat-shock transformation to make bacteria competent to take up plasmid DNA or we use lentiviral transduction (Lentivirus) to integrate genes in the genome of eukaryotic cells.

Cheers
Henrik


References:

Bacterial Transformation:
https://www.thermofisher.com/de/de/ho...
https://www.ncbi.nlm.nih.gov/pmc/arti...

Transduction:
https://www.ncbi.nlm.nih.gov/pmc/arti...
https://www.nature.com/articles/280080a0

Bacterial Conjugation:
https://www.ncbi.nlm.nih.gov/books/NB...

Cool & Short Blog article:
https://blog.addgene.org/plasmids-101...



Chapters:
0:00 Introduction
0:54 Transformation
2:43 Transduction
4:09 Conjugation